Collaboration with Institute Biology Leiden (IBL), Leiden University &
Medical Microbiology VUMC, Free University, Amsterdam
Stoop, EJM, Schipper, T., Rosendahl Huber, SK., Nezhinsky, AE., Verbeek, FJ., Gurcha, SS, Besra, GS., Vandenbroucke-Grauls, CMJE., Bitter, W., van der Sar, AM. (2011)
Zebrafish embryo screen for mycobacterial genes involved in initiation of granuloma formation reveals a novel ESX-1 component.
Disease Models and Mechanisms, 2011, 526-536. doi:10.1242/dmm.006676
The research/experimental procedures on bacterial load was performed at the VUMC, Amsterdam, the Netherlands. We collaborated in this project in designing and implementing software for the quantification of bacterial load.
In order to quantify bacterial load in zebrafish, fluorescent images of infected zebrafish embryos were analyzed with specially designed, dedicated software. For the analysis, 3 types of images are distinguished.
